OPTICAL MICROSCOPY (2D TO ND)

        • Analysis of fixed samples with multiple markers
        • Live cell monitoring
        • Spectral analysis and spectral separation
        • 3D imaging of big objects (by coupling slicing/acquisition, clearing, or light sheet microscopy)
           Wide-field epifluorescence and confocal microscopy, TIRF, light sheet microscopy

PHOTOMANIPULATION

        • Photoactivation of non-fluorescent molecules & photoconversion
        • LASER ablation, LASER-induced DNA damage
        • Uncaging
        • Optogenetics
          Wide-field microscopy, confocal microscopy (all visible LASER lines, 350nm)Photoactivation de molécules non fluorescentes & photoconversion

SUPER-RESOLUTION OPTICAL MICROSCOPY

        • 2D&3D multiplex super-resolution imaging
        (lateral resolution: 30-100nm; axial resolution: 80-300nm).
         • Surface imaging (atomic force microscopy)
            3D SIM, STED, 3D PALM/STORM & AFM

QUANTITATIVE DYNAMIC IMAGING  

        • Quantification of subcellular diffusion
        • Single Molecules Tracking
        • Identification of molecular interactions and subcellular characterization
           Ratiometric FRET imaging, BiFC, PLA, FLIM, RICS, FCS, FCCS, PIE, N&B

FACILITIES