STRUCTURAL ELECTRON MICROSCOPY OF ISOLATED HYDRATED-FROZEN PARTICLES
• Negative staining
• Quality control, concentration quantification
• Freezing and observation of hydrated-frozen samples
• AAnalysis, image treatment, and 3D reconstruction of the structure
(Cryo)microscopy, (cryo)tomography
PREPARATION OF BIOLOGICAL SAMPLES
• Preparation for scanning electron microscopy
• Fixation/cryofixation, inclusion, thin and ultra-thin sections
• Immunolabelling
CO2 critical point, metallization, immunogold
ELECTRON MICROSCOPY OF BIOLOGICAL SAMPLES
• Surface observation
• Structural analysis
• Immunogold labelling localization
• Correlative microscopy (fluorescence/EM)
MEB, TEM, STEM; tomography
FACILITIES