LIBRARY PREPARATION

        • Human and mouse ORFeome libraries and siRNA libraries, focused (human) or not     
        • Commercial chemical libraries (1280 compounds)
        • Academic chemical libraries CNE (640 compounds)
        • human CRISPR guides Lentiviruses

PREPARATION OF CELL CULTURES AND DISTRIBUTION

         Automated cell culture platform (distributors & pipettors)

MEDIUM & HIGH THROUGHPUT SCREENING

        • Ligand-receptor interaction    
        • Receptor internalization
        • Intracellular concentration of calcium, cAMP
        • Signal transduction (phosphorylated ERK, etc...)
        • Cell proliferation, cytotoxicity, adhesion, viability, invasion & migration
           Absorbance, luminescence, fluorimetry, TR-FRET, fluorescence polarization, label-free impedance

HIGH-CONTENT PHENOTYPIC SCREENING (MEDIUM & HIGH THROUGHPUT)  

        • Single-cell response, sub-populations
        • High throughput analysis of subcellular localization
        • Phenotypic analysis (apoptosis, blood vessel formation, colonies)
        • Cell structures (neurites, cytoskeleton, etc...)
        • Multiplex analysis
           High Content Screening, validation of protocol miniaturization, screening, image analysis, statistical validation of results

FACILITIES